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Lacibacter sediminis sp. nov., isolated from contaminated freshwater sediment.

Authors
  • Zhuo, Ye1, 2, 3
  • Jin, Chun-Zhi3
  • Jin, Feng-Jie1
  • Oh, Hee-Mock3
  • Lee, Hyung-Gwan3
  • Li, Taihua1
  • Jin, Long1
  • 1 Co-Innovation Centre for Sustainable Forestry in Southern China, College of Ecology and Environment, Nanjing Forestry University, Nanjing 210037, PR China. , (China)
  • 2 Department of Environmental biotechnology, KRIBB School of Biotechnology, Korea University of Science and Technology (UST), Daejeon, Republic of Korea. , (North Korea)
  • 3 Cell Factory Research Centre, Korea Research Institute of Bioscience & Biotechnology (KRIBB), Daejeon 34141, Republic of Korea. , (North Korea)
Type
Published Article
Journal
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Publisher
Microbiology Society
Publication Date
Nov 01, 2023
Volume
73
Issue
11
Identifiers
DOI: 10.1099/ijsem.0.006105
PMID: 37916707
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Two Gram-stain-negative bacterial strains, S13-6-6 and S13-6-22T, were isolated from sediment sample collected at a water depth of 4 m from Lake Hongze, Jiangsu Province, PR China. The cells of strains S13-6-6 and S13-6-22T were non-spore-forming, aerobic, non-motile and formed orange colonies on R2A agar. Comparative 16S rRNA gene sequence studies revealed a clear affiliation of the two strains with he phylum Bacteroidota, and revealed the highest pairwise sequence similarities with Lacibacter daechungensis H32-4T (97.8 %), Lacibacter cauensis NJ-8T (97.8 %), Lacibacter luteus TTM-7T (97.4 %) and Lacibacter nakdongensis SS2-56T (97.4 %). The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that the strains formed a clear phylogenetic lineage with the genus Lacibacter. The major fatty acids were identified as iso-C15 : 1G, iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) (>10 %), and the respiratory quinone was identified as menaquinone MK-7. The polar lipids consisted of phosphatidylethanolamine, two unidentified aminolipids, an unidentified phospholipid and six unidentified lipids. The genomic DNA G+C content was determined to be 40.2 mol% (HPLC) for strain S13-6-6 and 40.3 % (genome) for strain S13-6-22T. The combined genotypic and phenotypic data indicated that strains S13-6-6 and S13-6-22T represent a novel species of the genus Lacibacter, for which the name Lacibacter sediminis sp. nov. is proposed. The type strain is S13-6-22T (=CGMCC 1.17450T =JCM 35802T).

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