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Laboratory procedures for human in vitro fertilization.

Authors
  • Balaban, Basak
  • Sakkas, Denny
  • Gardner, David K
Type
Published Article
Journal
Seminars in reproductive medicine
Publication Date
Jul 01, 2014
Volume
32
Issue
4
Pages
272–282
Identifiers
DOI: 10.1055/s-0034-1375179
PMID: 24919026
Source
Medline
License
Unknown

Abstract

Successful and consistent outcomes in human in vitro fertilization (IVF) can be readily achieved by all IVF clinics through consideration and optimization of each procedure associated with the collection and processing of gametes, culminating in the resultant culture and transfer of healthy embryos. Furthermore, understanding the interactions between the individual components of the IVF cycle will assist when trouble-shooting possible problems in a laboratory which could have an adverse effect on cycle outcome. This article will review handling of oocytes and embryo culture, preparation of gametes for insemination and microinjection, selection of the most viable gametes and embryos, cryopreservation, and successful embryo transfer from the laboratory perspective.

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