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Label-free microscopy of mitotic chromosomes using the polarization orthogonality breaking technique

Authors
  • Desapogu, Rajesh1, 1, 2
  • Le Marchand, Gilles1, 2
  • Smith, Rebecca1
  • Ray, Paulami1
  • Gillier, Émilie1
  • Dutertre, Stéphanie1
  • Alouini, Mehdi1
  • Tramier, Marc1, 1
  • Huet, Sébastien1, 1, 3,
  • Fade, Julien1,
  • 1 Univ Rennes, France , (France)
  • 2 These authors contributed equally to this work
  • 3 Institut Universitaire de France, France , (France)
Type
Published Article
Journal
Biomedical Optics Express
Publisher
The Optical Society
Publication Date
Jul 28, 2021
Volume
12
Issue
8
Pages
5290–5304
Identifiers
DOI: 10.1364/BOE.426630
PMID: 34513257
PMCID: PMC8407833
Source
PubMed Central
Disciplines
  • Article
License
Unknown

Abstract

We report how a recently developed polarization imaging technique, implementing micro-wave photonics and referred to as orthogonality-breaking (OB) imaging, can be adapted on a classical confocal fluorescence microscope, and is able to provide informative polarization images from a single scan of the cell sample. For instance, the comparison of the images of various cell lines at different cell-cycle stages obtained by OB polarization microscopy and fluorescence confocal images shows that an endogenous polarimetric contrast arizes with this instrument on compacted chromosomes during cell division.

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