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L-phenylalanine production by double auxotrophic mutants of Arthrobacter globiformis.

Authors
  • Maiti, T K
  • Chatterjee, S P
Type
Published Article
Journal
Folia microbiologica
Publication Date
Jan 01, 1991
Volume
36
Issue
3
Pages
234–239
Identifiers
PMID: 1841857
Source
Medline
License
Unknown

Abstract

A number of tryptophan-plus-tyrosine double auxotrophs have been isolated from a glutamate producing Arthrobacter globiformis excreting L-phenylalanine by two-step mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine. For the three potent mutants tested the medium of Alföldi was found to be the best. The optimum tryptophan, tyrosine and biotin concentrations for phenylalanine production of these mutants were 0.5 mmol/L, 0.1 mmol/L and 5 micrograms/L, respectively. At these levels strain TT-39 yielded 2.6 g phenylalanine per L of medium in flask culture with glucose (350 mmol/L) and NH4Cl (60 mmol/L).

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