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The Kw recombinase, an integrase from Kluyveromyces waltii.

Authors
Type
Published Article
Journal
European journal of biochemistry / FEBS
Publication Date
Volume
248
Issue
3
Pages
903–912
Identifiers
PMID: 9342245
Source
Medline

Abstract

Site-specific recombinases of the integrase family share limited amino-acid-sequence similarity, but use a common reaction mechanism to recombine distinct DNA target sites. Here we report the characterisation of the Kw site-specific recombinase, encoded on the 2 mu-like plasmid pKWS1 from the yeast Kluyveromyces waltii. Using in vitro-translated Kw recombinase, we show that the protein is able to bind and to recombine its putative DNA target site. Recombination is conservative and the Kw target site has a spacer of seven base pairs. We show that Kw recombinase is able to mediate recombination in a mammalian cell line, thus, it has potential for use as a tool for genomic manipulation in heterologous systems.

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