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The Komodo dragon (Varanus komodoensis) genome and identification of innate immunity genes and clusters

Authors
  • van Hoek, Monique L.1
  • Prickett, M. Dennis2
  • Settlage, Robert E.3
  • Kang, Lin4
  • Michalak, Pawel4, 5, 6
  • Vliet, Kent A.7
  • Bishop, Barney M.8
  • 1 George Mason University, School of Systems Biology, Manassas, VA, 20110, USA , Manassas (United States)
  • 2 Università di Trieste, Dipartimento di Scienze della Vita-Edif. C11, Via Licio Giorgieri 1, Trieste, 34127, Italy , Trieste (Italy)
  • 3 Virginia Polytechnic Institute and State University, Advanced Research Computing, Blacksburg, VA, 24061, USA , Blacksburg (United States)
  • 4 Edward Via College of Osteopathic Medicine, Blacksburg, VA, 24060, USA , Blacksburg (United States)
  • 5 Virginia-Maryland College of Veterinary Medicine, Center for One Health Research, Blacksburg, VA, 24060, USA , Blacksburg (United States)
  • 6 University of Haifa, Institute of Evolution, Haifa, 3498838, Israel , Haifa (Israel)
  • 7 University of Florida, Department of Biology, Gainesville, Florida, FL, 32611, USA , Florida (United States)
  • 8 George Mason University, Department of Chemistry, Manassas, VA, 20110, USA , Manassas (United States)
Type
Published Article
Journal
BMC Genomics
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Aug 30, 2019
Volume
20
Issue
1
Identifiers
DOI: 10.1186/s12864-019-6029-y
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundWe report the sequencing, assembly and analysis of the genome of the Komodo dragon (Varanus komodoensis), the largest extant lizard, with a focus on antimicrobial host-defense peptides. The Komodo dragon diet includes carrion, and a complex milieu of bacteria, including potentially pathogenic strains, has been detected in the saliva of wild dragons. They appear to be unaffected, suggesting that dragons have robust defenses against infection. While little information is available regarding the molecular biology of reptile immunity, it is believed that innate immunity, which employs antimicrobial host-defense peptides including defensins and cathelicidins, plays a more prominent role in reptile immunity than it does in mammals. .ResultsHigh molecular weight genomic DNA was extracted from Komodo dragon blood cells. Subsequent sequencing and assembly of the genome from the collected DNA yielded a genome size of 1.6 Gb with 45x coverage, and the identification of 17,213 predicted genes. Through further analyses of the genome, we identified genes and gene-clusters corresponding to antimicrobial host-defense peptide genes. Multiple β-defensin-related gene clusters were identified, as well as a cluster of potential Komodo dragon ovodefensin genes located in close proximity to a cluster of Komodo dragon β-defensin genes. In addition to these defensins, multiple cathelicidin-like genes were also identified in the genome. Overall, 66 β-defensin genes, six ovodefensin genes and three cathelicidin genes were identified in the Komodo dragon genome.ConclusionsGenes with important roles in host-defense and innate immunity were identified in this newly sequenced Komodo dragon genome, suggesting that these organisms have a robust innate immune system. Specifically, multiple Komodo antimicrobial peptide genes were identified. Importantly, many of the antimicrobial peptide genes were found in gene clusters. We found that these innate immunity genes are conserved among reptiles, and the organization is similar to that seen in other avian and reptilian species. Having the genome of this important squamate will allow researchers to learn more about reptilian gene families and will be a valuable resource for researchers studying the evolution and biology of the endangered Komodo dragon.

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