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Knock-out mutation of eukaryotic initiation factor 4E2 (eIF4E2) confers resistance to pepper veinal mottle virus in tomato.

Authors
  • Moury, Benoît1
  • Lebaron, Caroline2
  • Szadkowski, Marion3
  • Ben Khalifa, Mekki4
  • Girardot, Grégory3
  • Bolou Bi, Bolou Antoine5
  • Koné, Daouda5
  • Nitiema, Léon W6
  • Fakhfakh, Hatem7
  • Gallois, Jean-Luc2
  • 1 Pathologie Végétale, INRA, 84140, Montfavet, France. Electronic address: benoit.mour[email protected] , (France)
  • 2 GAFL, INRA, 84140, Montfavet, France. , (France)
  • 3 Pathologie Végétale, INRA, 84140, Montfavet, France. , (France)
  • 4 Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie, Faculté des Sciences de Tunis, Université de Tunis El-Manar, Tunis, Tunisia; Faculté des Sciences de Bizerte, Zarzouna, Université de Carthage, Bizerte, Tunisia. , (Tunisia)
  • 5 Laboratoire de Physiologie Végétale, UFR Biosciences, Université Felix Houphouët-Boigny, Abidjan 22, BP 582, Cote d'Ivoire.
  • 6 Institut de l'Environnement et de Recherches Agricoles (INERA), 01 BP 476, Ouagadougou 01, Burkina Faso. , (Burkina Faso)
  • 7 Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie, Faculté des Sciences de Tunis, Université de Tunis El-Manar, Tunis, Tunisia. , (Tunisia)
Type
Published Article
Journal
Virology
Publisher
Elsevier
Publication Date
Oct 03, 2019
Volume
539
Pages
11–17
Identifiers
DOI: 10.1016/j.virol.2019.09.015
PMID: 31622792
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Translation initiation factors 4E (eIF4E) are the main source of resistance to potyvirus. We systematically assessed tomato single and double knock-out (KO) mutants of members of the eIF4E-coding gene family for resistance to Pepper veinal mottle virus (PVMV), a major constraint to tomato production. We show that the KO mutant of eIF4E2 has partial resistance to PVMV isolate IC, with plants harboring weak symptoms and low virus loads at the systemic level. The causal effect of eIF4E2 loss-of-function on resistance was confirmed on a progeny segregating for the KO mutation. The eIF4E2 KO mutant was resistant to six of the eight PVMV isolates tested and no resistance to other potyviruses was observed. This is the first evidence that mutation of eIF4E2 is in itself conferring resistance to a potyvirus and 3D protein modelling suggests that the eIF4E2 gene could be converted into a functional resistance allele. Copyright © 2019 Elsevier Inc. All rights reserved.

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