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Kinetics of 3H-thymidine label in rat liver regenerating after partial hepatectomy and after damage with carbon tetrachloride or silica.

Authors
Type
Published Article
Journal
Sborník vědeckých prací Lékařské fakulty Karlovy university v Hradci Králové
Publication Date
Volume
32
Issue
4
Pages
395–401
Identifiers
PMID: 2561578
Source
Medline
License
Unknown

Abstract

Rat liver DNA was labelled with [methyl-3H] thymidine after partial hepatectomy, carbon tetrachloride poisoning, or an intravenous injection of silica dust. Changes in DNA labelling were studied for 4 weeks after the single pulse. Total radioactivity incorporated into liver DNA after partial hepatectomy and after carbon tetrachloride administration remained on the same level when compared with that found after 1 h. DNA activity in liver of untreated rats and of rats treated with silica decreased by about 50% within the first 2 weeks and then remained on this level for the rest of the studied period. These differences may reflect the fact that hepatocytes that have a long life span are preferentially labelled in partially hepatectomized and CCl4-treated rats, while liver macrophages with a short half-life take up a large part of the label in intact rats and in rats treated with silica.

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