Affordable Access

Kinetic behaviour of human erythrocyte casein kinases. Effect of 2, 3-bisphosphoglycerate and heparin.

Authors
Type
Published Article
Journal
The Italian journal of biochemistry
Publication Date
Volume
32
Issue
3
Pages
174–188
Identifiers
PMID: 6581148
Source
Medline
License
Unknown

Abstract

The predominant cAMP-independent casein kinase CS isolated from human erythrocyte hemolysate and membrane casein kinase MS are inhibited to the same extent by 2,3-bisphosphoglycerate (2,3-DPG). However, under some conditions, both enzymes may appear to be unaffected or even stimulated by this prominent red cell metabolite, depending on casein and monovalent salt (NaCl, KCl) concentrations employed in the assay. In addition, both casein kinases CS and MS exhibit identical response to heparin, being unaffected by increasing concentrations of this mucopolysaccharide up to 400 ng/ml. On the contrary, the minor cytosolic casein kinase CTS, is inhibited by both 2,3-DPG and heparin under all conditions tested in the assay.

Statistics

Seen <100 times