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Kidney osteoclast factors and matrix metalloproteinase expression in a mice model of diet-induced obesity and diabetes.

Authors
  • Dos Santos-Macedo, Francine1
  • Martins Gregorio, Bianca2
  • Cardozo Paes-de-Almeida, Elan3
  • de Souza Mendonça, Leonardo4
  • de Souza Azevedo, Rebeca5
  • Fernandes-Santos, Caroline6
  • 1 Laboratório Multiusuário de Pesquisa Biomédica, Departamento de Ciências Básicas, Universidade Federal Fluminense, Instituto de Saúde de Nova Friburgo, RJ, Brazil. Electronic address: [email protected] , (Brazil)
  • 2 Unidade de Pesquisa Urogenital, Universidade do Estado do Rio de Janeiro, RJ, Brazil. Electronic address: [email protected] , (Brazil)
  • 3 Laboratório Multiusuário de Pesquisa Biomédica, Departamento de Ciências Básicas, Universidade Federal Fluminense, Instituto de Saúde de Nova Friburgo, RJ, Brazil. Electronic address: [email protected] , (Brazil)
  • 4 Laboratório Multiusuário de Pesquisa Biomédica, Departamento de Ciências Básicas, Universidade Federal Fluminense, Instituto de Saúde de Nova Friburgo, RJ, Brazil. Electronic address: [email protected] , (Brazil)
  • 5 Laboratório de Patologia Oral, Departamento de Formação Específica, Universidade Federal Fluminense, Instituto de Saúde de Nova Friburgo, RJ, Brazil. Electronic address: [email protected] , (Brazil)
  • 6 Laboratório Multiusuário de Pesquisa Biomédica, Departamento de Ciências Básicas, Universidade Federal Fluminense, Instituto de Saúde de Nova Friburgo, RJ, Brazil. Electronic address: [email protected] , (Brazil)
Type
Published Article
Journal
Pathology, research and practice
Publication Date
Sep 01, 2019
Volume
215
Issue
9
Pages
152517–152517
Identifiers
DOI: 10.1016/j.prp.2019.152517
PMID: 31262577
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The role of RANKL/RANK/OPG system on bone remodeling is well known, and there is evidence that it is also important to cardiovascular and kidney pathology, although the underlying mechanisms are not elucidated so far. Thus, we investigated in a mice model of diet-induced obesity and diabetes if renal histopathological changes are associated with the expression of RANKL/RANK/OPG system and matrix metalloproteinases (MMPs). Three months old C57BL/6 mice were fed with control (C) AIN93 M diet or high-fat high sucrose (HFHS) diets for 21 weeks (CEUA/UFF #647/15). The two groups presented weight gain, but it was higher in the HFHS group compared to the C group (+35%, P = 0.0001). The HFHS group also had increased epididymal, inguinal and retroperitoneal fat pad weight (+121%, P = 0.0006; +287%, P = 0.0007 and; +286%, P < 0.0001, respectively), and hyperglycemia (+43%, P = 0.02). The kidney of some HFHS fed mice displayed mononuclear inflammatory cell infiltrate (40%), perivascular fibrosis (20%), and focal tubule mineralization (20%). Glomeruli hypertrophy was not detected. Unexpectedly, OPG, RANK, MMP-2, and MMP-9 expression was not altered in HFHS groups (Western blot analysis). In conclusion, the expression of RANKL/RANK/OPG system proteins and MMPs was not influenced by diet-induced obesity and diabetes in the kidney of male C57BL/6 mice, although some adverse histopathological remodeling is noticed in the renal tissue. Copyright © 2019 Elsevier GmbH. All rights reserved.

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