How to Cite This Article Osorio M, Miranda J, González M, Mattar S: Anaplasma sp., Ehrlichia sp., and Rickettsia sp. in ticks: A high risk for public health in Ibagué, Colombia. Abstract The Order Rickettsiales comprises intracellular bacteria, including Rickettsiaceae and Anaplasmataceae; members of these families cause zoonotic diseases transmitted by ticks. The aim of this study was to make a detection of Anaplasma, Ehrlichia and Rickettsia in ticks of the Ixodidae (Acari: Ixodidae) family collected from domestic animals in Ibagué, Colombia. Ticks were collected from dogs, horses and cattle, classified taxonomically, and then subjected to DNA extraction. To detect Anaplasma sp., Ehrlichia sp., and Rickettsia sp. we carried out a conventional PCR to detect the gltA gene for Rickettsia, 16S rRNA for Anaplasma and Ehrlichia, and the dsb gene for Ehrlichia. The phylogenetic trees were constructed with the Neighbor-Joining method. A total of 1.247 ticks, mainly R. microplus, R. sanguineus and D. nitens, were collected. Anaplasma and Ehrlichia were detected in thirteen samples. The sequences showed a genetic similarity with E. canis, E. mineirensis, A. phagocytophilum, and A. marginale. No Rickettsia was found. This is the first time that active circulation of Anaplasma and Ehrlichia is demonstrated in Ibagué. Both pathogens are important because can produce economic losses in animals and humans disease. This finding will contribute to the implementation of early epidemiological alerts, as well as the design of measures to prevent and control diseases in the region.