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Karyotypes and Sex Chromosomes in Two Australian Native Freshwater Fishes, Golden Perch ( Macquaria ambigua ) and Murray Cod ( Maccullochella peelii ) (Percichthyidae)

Authors
  • Shams, Foyez1
  • Dyer, Fiona1
  • Thompson, Ross1
  • Duncan, Richard P.1
  • Thiem, Jason D.2
  • Majtánová, Zuzana3
  • Ezaz, Tariq1
  • 1 Institute for Applied Ecology, Faculty of Science and Technology, University of Canberra, Canberra, Australian Capital Territory 2617, Australia
  • 2 Department of Primary Industries, Narrandera Fisheries Centre, Narrandera, New South Wales 2700, Australia
  • 3 Institute of Animal Physiology and Genetics, CAS, p.r.i., 277 21 Liběchov, Czech Republic
Type
Published Article
Journal
International Journal of Molecular Sciences
Publisher
MDPI AG
Publication Date
Aug 30, 2019
Volume
20
Issue
17
Identifiers
DOI: 10.3390/ijms20174244
PMID: 31480228
PMCID: PMC6747191
Source
PubMed Central
Keywords
License
Green

Abstract

Karyotypic data from Australian native freshwater fishes are scarce, having been described from relatively few species. Golden perch ( Macquaria ambigua ) and Murray cod ( Maccullochella peelii ) are two large-bodied freshwater fish species native to Australia with significant indigenous, cultural, recreational and commercial value. The arid landscape over much of these fishes’ range, coupled with the boom and bust hydrology of their habitat, means that these species have potential to provide useful evolutionary insights, such as karyotypes and sex chromosome evolution in vertebrates. Here we applied standard and molecular cytogenetic techniques to characterise karyotypes for golden perch and Murray cod. Both species have a diploid chromosome number 2 n = 48 and a male heterogametic sex chromosome system (XX/XY). While the karyotype of golden perch is composed exclusively of acrocentric chromosomes, the karyotype of Murray cod consists of two submetacentric and 46 subtelocentric/acrocentric chromosomes. We have identified variable accumulation of repetitive sequences (AAT)10 and (CGG)10 along with diverse methylation patterns, especially on the sex chromosomes in both species. Our study provides a baseline for future cytogenetic analyses of other Australian freshwater fishes, especially species from the family Percichthyidae, to better understand their genome and sex chromosome evolution.

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