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The Coordinated Upregulated Expression of Genes Involved in MEP, Chlorophyll, Carotenoid and Tocopherol Pathways, Mirrored the Corresponding Metabolite Contents in Rice Leaves during De-Etiolation.

Authors
  • Jin, Xin1, 2, 3
  • Baysal, Can3
  • Drapal, Margit4
  • Sheng, Yanmin5
  • Huang, Xin3
  • He, Wenshu3
  • Shi, Lianxuan6
  • Capell, Teresa3
  • Fraser, Paul D4
  • Christou, Paul3, 7
  • Zhu, Changfu3, 5
  • 1 Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China. , (China)
  • 2 College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China. , (China)
  • 3 Department of Plant Production and Forestry Science, University of Lleida-Agrotecnio CERCA Center, Av. Alcalde Rovira Roure, 191, 25198 Lleida, Spain. , (Spain)
  • 4 Biochemistry, School of Life Sciences and Environment, Royal Holloway University of London, Egham Hill, Egham, Surrey TW20 0EX, UK.
  • 5 School of Life Sciences, Changchun Normal University, Changchun 130032, China. , (China)
  • 6 School of Life Sciences, Northeast Normal University, Changchun 130024, China. , (China)
  • 7 ICREA, Catalan Institute for Research and Advanced Studies, Passeig Lluís Companys 23, 08010 Barcelona, Spain. , (Spain)
Type
Published Article
Journal
Plants
Publisher
MDPI AG
Publication Date
Jul 16, 2021
Volume
10
Issue
7
Identifiers
DOI: 10.3390/plants10071456
PMID: 34371659
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Light is an essential regulator of many developmental processes in higher plants. We investigated the effect of 4-hydroxy-3-methylbut-2-enyl diphosphate reductase 1/2 genes (OsHDR1/2) and isopentenyl diphosphate isomerase 1/2 genes (OsIPPI1/2) on the biosynthesis of chlorophylls, carotenoids, and phytosterols in 14-day-old etiolated rice (Oyza sativa L.) leaves during de-etiolation. However, little is known about the effect of isoprenoid biosynthesis genes on the corresponding metabolites during the de-etiolation of etiolated rice leaves. The results showed that the levels of α-tocopherol were significantly increased in de-etiolated rice leaves. Similar to 1-deoxy-D-xylulose-5-phosphate synthase 3 gene (OsDXS3), both OsDXS1 and OsDXS2 genes encode functional 1-deoxy-D-xylulose-5-phosphate synthase (DXS) activities. Their expression patterns and the synthesis of chlorophyll, carotenoid, and tocopherol metabolites suggested that OsDXS1 is responsible for the biosynthesis of plastidial isoprenoids in de-etiolated rice leaves. The expression analysis of isoprenoid biosynthesis genes revealed that the coordinated expression of the MEP (2-C-methyl-D-erythritol 4-phosphate) pathway, chlorophyll, carotenoid, and tocopherol pathway genes mirrored the changes in the levels of the corresponding metabolites during de-etiolation. The underpinning mechanistic basis of coordinated light-upregulated gene expression was elucidated during the de-etiolation process, specifically the role of light-responsive cis-regulatory motifs in the promoter region of these genes. In silico promoter analysis showed that the light-responsive cis-regulatory elements presented in all the promoter regions of each light-upregulated gene, providing an important link between observed phenotype during de-etiolation and the molecular machinery controlling expression of these genes.

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