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Kallikrein-Related Peptidase mRNA Expression in Adenoid Cystic Carcinoma of Salivary Glands: A Polymerase Chain Reaction Study.

Authors
  • Kerr, Zachary1
  • Hayter, Alyssa2
  • Khan, Zia2
  • Darling, Mark3
  • 1 Interface Oral Surgery, London, ON, Canada. , (Canada)
  • 2 Pathology and Laboratory Medicine, Schulich School of Medicine and Dentistry, University of Western Ontario, 1151 Richmond St, London, ON, N5X 0H4, Canada. , (Canada)
  • 3 Pathology and Laboratory Medicine, Schulich School of Medicine and Dentistry, University of Western Ontario, 1151 Richmond St, London, ON, N5X 0H4, Canada. [email protected] , (Canada)
Type
Published Article
Journal
Head and neck pathology
Publication Date
Sep 01, 2020
Volume
14
Issue
3
Pages
577–587
Identifiers
DOI: 10.1007/s12105-019-01076-4
PMID: 31515705
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Kallikrein-related peptidases (KLKs) are a group of 15 serine proteases implicated in a variety of biological processes. Aberrant expression of KLKs has been associated with the development of certain cancers. However, the role of KLKs in salivary tumors has not been extensively studied. This study evaluated the expression of KLKs in both adenoid cystic carcinoma (ACC) and normal salivary gland tissue. We isolated total RNA from 39 formalin-fixed, paraffin-embedded samples, which included 24 ACCs and 15 normal salivary gland tissues. Complementary DNA, synthesized by reverse transcription, was combined with gene specific kallikrein primers (KLK1-KLK15) to allow for quantitative real-time PCR. Data was normalized to a β-actin housekeeping gene. Relative quantification analysis was performed using the ΔCq method. KLK1-KLK15 expression was observed in both tissue types. However, KLK1, KLK8, KLK11, and KLK14 were found to be downregulated in ACC. We propose that this may represent a multi-parametric panel providing diagnostic and prognostic information.

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