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Jaw osteosarcoma models in mice: first description

Authors
  • Bertin, Hélios
  • Guilho, Romain
  • Brion, Régis
  • Amiaud, Jérôme
  • Battaglia, Séverine
  • Moreau, Anne
  • Brouchet-Gomez, Anne
  • Longis, Julie
  • Piot, Benoit
  • Heymann, Dominique
  • Corre, Pierre
  • Rédini, Françoise
Type
Published Article
Journal
Journal of Translational Medicine
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Feb 27, 2019
Volume
17
Issue
1
Identifiers
DOI: 10.1186/s12967-019-1807-5
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundOsteosarcoma (OS) is the most common cancer of bone. Jaw osteosarcoma (JOS) is rare and it differs from other OS in terms of the time of occurrence (two decades later) and better survival. The aim of our work was to develop and characterize specific mouse models of JOS.MethodsSyngenic and xenogenic models of JOS were developed in mice using mouse (MOS-J) and human (HOS1544) osteosarcoma cell lines, respectively. An orthotopic patient-derived xenograft model (PDX) was also developed from a mandibular biopsy. These models were characterized at the histological and micro-CT imaging levels, as well as in terms of tumor growth and metastatic spread.ResultsHomogeneous tumor growth was observed in both the HOS1544 and the MOS-J JOS models by injection of 0.25 × 106 and 0.50 × 106 tumor cells, respectively, at perimandibular sites. Histological characterization of the tumors revealed features consistent with high grade conventional osteosarcoma, and the micro-CT analysis revealed both osteogenic and osteolytic lesions. Early metastasis was encountered at day 14 in the xenogenic model, while there were no metastatic lesions in the syngenic model and in the PDX models.ConclusionWe describe the first animal model of JOS and its potential use for therapeutic applications. This model needs to be compared with the usual long-bone osteosarcoma models to investigate potential differences in the bone microenvironment.

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