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Jasmonic acid and hydrogen sulfide modulate transcriptional and enzymatic changes of plasma membrane NADPH oxidases (NOXs) and decrease oxidative damage in Oryza sativa L. during thiocyanate exposure.

Authors
  • Yu, Xiao-Zhang1
  • Chu, Yun-Peng2
  • Zhang, Hua2
  • Lin, Yu-Juan2
  • Tian, Peng2
  • 1 College of Environmental Science & Engineering, Guilin University of Technology, 541004, Guilin, PR China. [email protected] , (China)
  • 2 College of Environmental Science & Engineering, Guilin University of Technology, 541004, Guilin, PR China. , (China)
Type
Published Article
Journal
Ecotoxicology (London, England)
Publication Date
Oct 01, 2021
Volume
30
Issue
8
Pages
1511–1520
Identifiers
DOI: 10.1007/s10646-020-02266-5
PMID: 32821975
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

It is evident that the plasma membrane NADPH oxidases (NOXs) play an important role in the generation of superoxide radicals (O2-•) in plants during defense responses. This study was to clarify activation of NOXs in oxidative damage in Oryza sativa during SCN- exposure, particularly in the roles of jasmonic acid (JA) and hydrogen sulfide (H2S) on transcriptional and enzymatic changes of NOXs. Results indicated that enzymatic activity of NOXs in both roots and shoots was significantly enhanced during SCN- exposure, whereas the application of JA and H2S donor (NaHS) significantly repressed NOXs activity in SCN-treated rice seedlings. Similarly, ROS analysis showed that SCN- exposure elevated the content of O2-•, hydrogen peroxide (H2O2) and malondialdehyde (MDA) in rice tissues significantly, whereas decreases in O2-• and H2O2 were detected in roots and shoots of SCN-treated rice seedlings due to application of JA and NaHS. PCR analysis revealed different expression patterns of 7 plasma membrane-localized NOX genes in rice roots and shoots against SCN- exposure, suggesting that various isogenes of NOXs might regulate and determine activity of NOXs in rice organs. In conclusion, SCN- exposure was able to trigger activation of NOXs effectively, and led to oxidative damage and lipid peroxidation; the effects of JA and NaHS on inactivation of NOXs was evident and tissue specific, which in turn modulated ROS accumulation in rice plants. © 2020. Springer Science+Business Media, LLC, part of Springer Nature.

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