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Isolation and subunit structure of the xylanosome complex produced by Actinotalea fermentans JCM9966.

Authors
  • Dou, Tong-Yi1
  • Chen, Jing2
  • Liu, Wan-Jun2
  • Wang, Lu3
  • 1 School of Life Science and Medicine, Dalian University of Technology, Dagong Road No.2, LiaoDongWan New District, Panjin, 124221, China. [email protected] , (China)
  • 2 School of Life Science and Medicine, Dalian University of Technology, Dagong Road No.2, LiaoDongWan New District, Panjin, 124221, China. , (China)
  • 3 Key Laboratory of Xinjiang Phytomedicine Resource and Utilization, Ministry of Education, Pharmacy School of Shihezi University, Xinjiang Uygur, 832000, China. , (China)
Type
Published Article
Journal
Biotechnology Letters
Publisher
Springer-Verlag
Publication Date
Nov 12, 2019
Identifiers
DOI: 10.1007/s10529-019-02761-8
PMID: 31720977
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Xylanosomes, also known as hemicellulosomes, are hemicellulose-degrading nano-scale multienzyme complexes produced by some Firmicutes, Actinobacteria, and Fungi. Here we report the isolation of the MECs produced by Actinotalea fermentas JCM9966, as well as the functional studies and subunit structure revealed by proteomic identifications. The isolated MECs here shows similar particle size with the xylanosomes produced by C. cellulans F16, have several conserved multi-domain proteins, while differ significantly in enzymatic activities and low molecular weight subunit compositions, indicating diverse capability as well as bias in degrading hemicelluloses.

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