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[Isolation, structure elucidation and synthesis of a hexapeptide from the hemodialysate of uremic patients].

Authors
  • Bovermann, G
  • Rautenstrauch, H
  • Seybold, G
  • Jung, G
Type
Published Article
Journal
Hoppe-Seyler's Zeitschrift fur physiologische Chemie
Publication Date
Oct 01, 1982
Volume
363
Issue
10
Pages
1187–1202
Identifiers
PMID: 7141402
Source
Medline
Language
German
License
Unknown

Abstract

Lyophilised hemodialysates of uremic patients were fractionated on Sephadex G-15 for investigation of potential uremic toxins. One of the oligopeptides found in the fraction of postulated toxins was purified to homogeneity by gel, reversed-phase and adsorption chromatography and by high-performance liquid chromatography. Dansyl Edman degradation revealed the sequence Ala-Phe-Phe-Gly-Glu, which was synthesized by the solid-phase method. The comparison of synthetic and natural hexapeptide by chromatographic methods, mass spectrometry, 1H- and 13C-nuclear magnetic resonance proved the correct sequence. The peptide showed only weak activity in immunological test systems and in the lymphocyte proliferation test.

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