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Isolation of a stable enzyme.14CO2.Mg2+.carboxyarabinitol bisphosphate complex with ribulosebisphosphate carboxylase from Chromatium vinosum.

Authors
  • Brown, H M
  • Chollet, R
Type
Published Article
Journal
Journal of bacteriology
Publication Date
Mar 01, 1982
Volume
149
Issue
3
Pages
1159–1161
Identifiers
PMID: 6801011
Source
Medline
License
Unknown

Abstract

Higher plant-type ribulosebisphoshate carboxylase from Chromatium vinosum formed a stable, nonexchangeable complex with activator 14CO2 in the presence of Mg2+ and 2-carboxyarabinitol bisphosphate, an analog of the proposed transition-state intermediate. The response of the procaryotic enzyme to this analog was indistinguishable from that of the higher-plant carboxylase, which should permit comparative analysis of the activator site amino acid sequence in the two proteins.

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