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Isolation and quality control of functional mitochondria.

Authors
Type
Published Article
Journal
Methods in Molecular Biology
1064-3745
Publication Date
Volume
1264
Pages
9–23
Identifiers
DOI: 10.1007/978-1-4939-2257-4_2
PMID: 25630999
Source
Medline

Abstract

Numerous protocols are available being adapted for different cell or tissue types allowing isolation of pure mitochondria trying to preserve their "structural and functional" integrity. In this chapter we intend to provide a more general framework introducing differential isopycnic density gradient centrifugation strategy with a special focus sensitizing for the specific challenges coming along with this method and how to obtain "functional," enriched, "intact" mitochondria. Due to the fact that in any study dealing with these organelles standardized processing is mandatory. Here we describe a strategy addressing quality control of prepared intact mitochondria. The quality control should be an integrated part of all isolation processes. The underlying protocol should be seen as starting point and has to be carefully adjusted to cover different sample types used for the diverse research questions.

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