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[Isolation, purification and identification of metallthionein from strain BD 101 of Saccharomyces cerevisiae].

Authors
  • Lin, Z
  • Chang, L
Type
Published Article
Journal
Wei sheng wu xue bao = Acta microbiologica Sinica
Publication Date
Aug 01, 1998
Volume
38
Issue
4
Pages
289–294
Identifiers
PMID: 12549417
Source
Medline
License
Unknown

Abstract

Selection of Saccharomyces cerevisiae BD101 having resistance to metal ions and producing metallothionein by inducer from Saccharomyces. These proteins were isolated and purified by Sephadex G-50 and subsequent DEAE Sepharose CL-4B, then Sephadex G-25 for desalination. There were two iso-MTs for Cu-induced. The, molecular weights were 7 kD and had 60 amino acids. Both Cu-MTs were rich in Cysteine (10%). 4 atom Cu/6 mole Cys/mole Cu-MTs.

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