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Isolation and proteomic analysis of the halotolerant alga Dunaliella salina flagella using shotgun strategy.

Authors
Type
Published Article
Journal
Molecular Biology Reports
1573-4978
Publisher
Springer-Verlag
Publication Date
Volume
37
Issue
2
Pages
711–716
Identifiers
DOI: 10.1007/s11033-009-9563-x
PMID: 19437132
Source
Medline
License
Unknown

Abstract

Previous studies have demonstrated that flagella/cilia are critical organelles and play diverse roles of motility, sensory perception and development in many eukaryotic cells. However, there is very little information available about flagella composition in Dunaliella salina, a halotolerant, unicellular biflagellate green alga. In the present study, we used strategy of shotgun proteomics to identify flagella proteins after flagella were released and collected from D. salina. A total of 520 groups of proteins were identified under a stringent filter condition (Xcorr > or =1.9, > or =2.2 and > or =3.75; DeltaCn >/= 0.1). In addition to six kinds of known flagella proteins, the putative flagella proteins of D. salina identified by one or more peptides are abundant in signaling, cell division, metabolism, etc. The findings provide guidance for further studies to elucidate the roles of these proteins in the function and assembly of this organelle in microalgae.

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