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Isolation and partial characterization of the most immunologically reactive antigen from Mycobacterium tuberculosis H37Ra culture filtrate.

Authors
  • Reich, M
  • Affronti, L F
  • Wright, G L Jr
Type
Published Article
Journal
Tubercle
Publication Date
Jun 01, 1982
Volume
63
Issue
2
Pages
99–106
Identifiers
PMID: 6817485
Source
Medline
License
Unknown

Abstract

The culture filtrate of Mycobacterium tuberculosis H37Ra was fractionated by ammonium sulphate precipitation, diethyl aminoethyl (DEAE) cellulose-Sephadex A-50 column chromatography, preparative isoelectric focusing and polyacrylamide gel electrophoresis to isolate the most antigenic tuberculoprotein. One protein, designated P2, was isolated and determined by immunodiffusion to be the most immunologically reactive antigen present in H37Ra culture filtrate. This protein had a molecular weight of about 22 000, a sedimentation constant of 2.2S and was present in Seibert's A, B, and C proteins.

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