Yamagishi, Takayoshi (Kanazawa University, Kanazawa, Japan), Shoichi Ishida, and Shoki Nishida. Isolation of toxigenic strains of Clostridium perfringens from soil. J. Bacteriol. 88:646–652. 1964—Strains of Clostridium perfringens were isolated from soil specimens heated at various temperatures for different lengths of time and were toxigenically examined. When higher temperatures were used, fewer toxigenic strains were isolated. Toxigenic strains could not be obtained from soil specimens heated at 100 C for 60 min. All toxigenic strains were killed by heating at 100 C for 10 min; strains that were resistant to these conditions were found to be nontoxigenic. These findings suggest that sporulating ability is closely linked to toxigenicity.