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Isolation of the Bacteriophage DinoHI from Dichelobacter nodosus and its Interactions with other Integrated Genetic Elements.

Authors
  • Cheetham, Brian F1
  • Parker, Dane
  • Bloomfield, Garry A
  • Shaw, Bruce E
  • Sutherland, Megan
  • Hyman, Jessica A
  • Druitt, Jenifer
  • Kennan, Ruth M
  • Rood, Julian I
  • Katz, Margaret E
  • 1 Molecular and Cellular Biology, University of New England, Armidale, NSW, 2351, Australia.
Type
Published Article
Journal
The Open Microbiology Journal
Publisher
Bentham Science
Publication Date
2008
Volume
2
Pages
1–9
Identifiers
DOI: 10.2174/1874285800802010001
PMID: 19088904
Source
Medline
License
Unknown

Abstract

The Gram-negative anaerobic pathogen Dichelobacter nodosus carries several genetic elements that integrate into the chromosome. These include the intA, intB, intC and intD elements, which integrate adjacent to csrA and pnpA, two putative global regulators of virulence and the virulence-related locus, vrl, which integrates into ssrA. Treatment of D. nodosus strains with ultraviolet light resulted in the isolation of DinoHI, a member of the Siphoviridae and the first bacteriophage to be identified in D. nodosus. Part of the DinoHI genome containing the packaging site is found in all D. nodosus strains tested and is located at the end of the vrl, suggesting a role for DinoHI in the transfer of the vrl by transduction. Like the intB element, the DinoHI genome contains a copy of regA which has similarity to the repressors of lambdoid bacteriophages, suggesting that the maintenance of DinoHI and the intB element may be co-ordinately controlled.

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