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Isolation of a Pure Dextranase from Penicillium funiculosum

Authors
  • L. Chaiet
  • A. J. Kempf
  • R. Harman
  • E. Kaczka
  • R. Weston
  • K. Nollstadt
  • F. J. Wolf
Publication Date
Sep 01, 1970
Source
PMC
Keywords
Disciplines
  • Biology
License
Unknown

Abstract

A dextranase, produced by Penicillium funiculosum, was purified 1,000-fold to yield the enzyme which was demonstrated by gel electrophoresis and electrofocusing to be a homogeneous protein. The purification method included acetone partition, ammonium sulfate fractionation, gel filtration, iron defecation and precipitation, and diethylaminoethyl-cellulose chromatography. The pure enzyme was also obtained by preparative gel electrophoresis. Gel-permeation chromatography indicates a molecular weight of 41,000. An isoelectric pH of 4.6 was established by electrofocusing. A 1-mg amount of the enzyme hydrolyzes a dextran substrate to yield 27,000 isomaltose reducing units in 2 hr.

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