Affordable Access

Access to the full text

Isolation and Mycotaxonomic Characterization of Colletotrichum lillacola: A Novel Species Causing Anthracnose of Bergenia ligulata

Authors
  • Dar, Rafiq Ahmad1
  • Rai, Akhila Nand1
  • Shiekh, Imtiyaz Ahmad2
  • 1 Dr. HS Gour Central University, Mycology and Plant Pathology Laboratory, Department of Botany, Sagar, India , Sagar (India)
  • 2 Dr. HS Gour Central University, Mushroom Biology Laboratory, Department of Botany, Sagar, India , Sagar (India)
Type
Published Article
Journal
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
Publisher
Springer India
Publication Date
Sep 02, 2017
Volume
88
Issue
4
Pages
1615–1620
Identifiers
DOI: 10.1007/s40011-017-0903-7
Source
Springer Nature
Keywords
License
Yellow

Abstract

Anthracnose symptoms were observed in the samples collected from the 39 infected plants of Bergenia ligulata (Wall) (family Saxifragaceae) along the Himalayan mountain ranges of India. Fungal isolates were recovered directly from the structures present on the lesions. Purified DNA from each isolate was amplified using the random amplified polymorphic DNA technique viz. recruiting the ITS1 and ITS4 specific primers. Amplification products visualized on agarose gel showed specific band pattern for each isolate. The mycotaxonomic characterization and phylogenetic interpretations showed the emergence of fungal species, Colletotrichum lillacola sp.nov. with genetic variance of 0.5 in internal transcribed spacer (ITS) region of rDNA from closely related species under geographically distinct region. The pathogen is having a lethal effect on the B. ligulata populations in India and solid containment measures should be imposed to restrict its spread throughout in its natural range ecologically, economically and pharmacologically as a precious host.

Report this publication

Statistics

Seen <100 times