Affordable Access

deepdyve-link
Publisher Website

Isolation and Molecular Detection of Newcastle Disease Virus from Field Outbreaks in Chickens in Central Ethiopia

Authors
  • Worku, Takele
  • Dandecha, Morka
  • Shegu, Deraje
  • Aliy, Abde
  • Negessu, Demessa
Type
Published Article
Journal
Veterinary Medicine : Research and Reports
Publisher
Dove
Publication Date
Apr 19, 2022
Volume
13
Pages
65–73
Identifiers
DOI: 10.2147/VMRR.S352727
PMID: 35469130
PMCID: PMC9034857
Source
PubMed Central
Keywords
Disciplines
  • Original Research
License
Unknown

Abstract

Background Newcastle disease is a major viral disease of poultry. The virus is a major problem for chickens in Ethiopia and there is a scarcity of updated information on the virological and molecular status of confirmation of Newcastle disease outbreak cases in the country. Methods Newcastle disease outbreaks were investigated from February 2021 to October 2021 in central Ethiopia to isolate and detect the virus by cell culture and reverse transcriptase PCR. A total of 44 pooled tissue specimens were sampled from sick and recently dead chickens showing typical clinical signs of Newcastle disease. Virus isolation were performed using DF-1 cells and detection of the virus was done by real-time PCR. Results Out of 44 collected tissue samples, 38.63% (17/44) were positive on DF-1 cells. The result shows 17 of the clinically sick and dead chickens were positive for the virus by reverse transcriptase polymerase chain reaction. Based on the sample type, 54.54% (6/11) of the brain samples, 36.36% (4/11) of the intestines, 54.54% (6/11) of lung and trachea, 9% (1/11) of pooled liver, kidney, heart, and spleen samples were positive. Viruses were isolated in the proportions 37.5% (6/16), 25% (2/8), 50% (2/4), 25% (1/4), 50% (2/4) and 50% (4/8) from Sebeta, Bishoftu, Sululta, Nifas Silk, Kolfe and Yeka, respectively. Conclusion This study showed that Newcastle disease is a major viral disease causing death of chickens in the study area. Therefore, any control approach should focus on the appropriate characterization of the virus strain causing the outbreak in the study area.

Report this publication

Statistics

Seen <100 times