Affordable Access

Isolation of macrophages from human placenta.

Authors
  • Uren, S
  • Boyle, W
Type
Published Article
Journal
Journal of Immunological Methods
Publisher
Elsevier
Publication Date
Apr 08, 1985
Volume
78
Issue
1
Pages
25–34
Identifiers
PMID: 3981018
Source
Medline
License
Unknown

Abstract

Human placentae have been extracted with combinations of enzymes to optimize the release of mononuclear phagocytes. A mixture of trypsin-DNAase used in sequential extraction was found to provide the best yield of adherent cells which were stable in culture. The majority of adherent cells exhibited phagocytic function and expression of receptor for IgG-Fc (FcR). Subsequent studies established that these functions were co-expressed by the same cells. The FcR+ cells were also shown by immunofluorescence with monoclonal antibodies to display monocyte-macrophage distinctive antigens and class I and class II MHC antigens. The placenta has thus been shown to provide a rich source of class II-positive macrophages suitable for immunological studies.

Report this publication

Statistics

Seen <100 times