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Isolation of human monoclonal antibodies binding to B fragment of diphtheria toxin.

Authors
  • Gupta, K C
  • Agha, R
  • Santos, E
  • Brodeur, B R
Type
Published Article
Journal
Human antibodies and hybridomas
Publication Date
Jan 01, 1992
Volume
3
Issue
1
Pages
25–31
Identifiers
PMID: 1576320
Source
Medline
License
Unknown

Abstract

Human tonsillar lymphocytes immunized in vitro with diphtheria toxoid were fused with the heteromyeloma cell line SP2/HPT. Hybrids were selected on hypoxanthine-thymidine aminopterin (HAT) medium and assayed for antibody production both by ELISA and Western blotting. Four stable hybridoma cell lines producing 3-7 micrograms/mL of human IgG1 antibodies were isolated. In Western blotting studies these antibodies show binding to the B fragment of diphtheria toxin. Cells from the subclone P2-13/G35 were injected i.p. in pristane-primed nude mice, and ascites fluid was collected after 4-6 weeks. The monoclonal antibody neutralized the cytotoxicity of diphtheria toxin in the Vero cell assay system. This human monoclonal antibody may have a potential in the immunotherapy of diphtheric disease.

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