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Isolation and growth characteristics of psittacine viruses in chicken embryos.

Authors
  • Hitchner, S B
  • Hirai, K
Type
Published Article
Journal
Avian diseases
Publication Date
Jan 01, 1979
Volume
23
Issue
1
Pages
139–147
Identifiers
PMID: 485999
Source
Medline
License
Unknown

Abstract

Eight viruses were isolated in embryonating eggs, from psittacine birds comprising a cockatiel, a budgerigar, 3 parrots, a love bird, and 2 rosellas. Initial attempts at isolation used 3 routes of embryo inoculation: yolk sac, allantoic sac, and chorioallantoic membrane (CAM). The most sensitive route was determined for 6 of the isolates by making comparative titrations by yolk sac, allantoic sac, and CAM routes of inoculation. In growth-curve studies of 6 of the isolates, virus concentration was determined daily for 5-6 days postinoculation in the CAM, allantoamnionic fluid, and liver. Peak virus concentrations appeared in about 72 hours. The embryonic lesions observed are described. Only one of the isolates hemagglutinated fowl red blood cells.

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