Isolation of Extracellular Vesicles from Subventricular Zone Neural Stem Cells.
Department of Biological Sciences, Clemson University, Clemson, SC, USA.
Department of Biological Sciences, Clemson University, Clemson, SC, USA. [email protected]
- Published Article
Methods in molecular biology (Clifton, N.J.)
- Publication Date
Jan 01, 2019
The neonatal subventricular zone (SVZ) is a neurogenic niche that contains neural stem cells (NSCs). NSCs release particles called extracellular vesicles (EVs) that contain biological material. EVs are transferred to cells, including immune cells in the brain called microglia. A standard approach to identify EV functions is to isolate and transplant EVs. Here, a detailed protocol is provided that will allow one to culture neonatal SVZ NSCs and to isolate, label, and transplant EVs. The protocol will permit careful and thorough examination of EVs in a wide range of physiological and pathophysiological conditions.
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This record was last updated on 03/30/2020 and may not reflect the most current and accurate biomedical/scientific data available from NLM.
The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/30244437