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Isolation of Extracellular Vesicles from Subventricular Zone Neural Stem Cells.

Authors
  • Morton, Mary C1
  • Neckles, Victoria N1
  • Feliciano, David M2
  • 1 Department of Biological Sciences, Clemson University, Clemson, SC, USA.
  • 2 Department of Biological Sciences, Clemson University, Clemson, SC, USA. [email protected]
Type
Published Article
Journal
Methods in molecular biology (Clifton, N.J.)
Publication Date
Jan 01, 2019
Volume
2002
Pages
75–85
Identifiers
DOI: 10.1007/7651_2018_183
PMID: 30244437
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The neonatal subventricular zone (SVZ) is a neurogenic niche that contains neural stem cells (NSCs). NSCs release particles called extracellular vesicles (EVs) that contain biological material. EVs are transferred to cells, including immune cells in the brain called microglia. A standard approach to identify EV functions is to isolate and transplant EVs. Here, a detailed protocol is provided that will allow one to culture neonatal SVZ NSCs and to isolate, label, and transplant EVs. The protocol will permit careful and thorough examination of EVs in a wide range of physiological and pathophysiological conditions.

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