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Isolation of chromosome 21-specific yeast artificial chromosomes from a total human genome library.

Authors
  • Chumakov, I M
  • Le Gall, I
  • Billault, A
  • Ougen, P
  • Soularue, P
  • Guillou, S
  • Rigault, P
  • Bui, H
  • De Tand, M F
  • Barillot, E
Type
Published Article
Journal
Nature genetics
Publication Date
Jun 01, 1992
Volume
1
Issue
3
Pages
222–225
Identifiers
PMID: 1303240
Source
Medline
License
Unknown

Abstract

A new approach for the isolation of chromosome-specific subsets from a human genomic yeast artificial chromosome (YAC) library is described. It is based on the hybridization with an Alu polymerase chain reaction (PCR) probe. We screened a 1.5 genome equivalent YAC library of megabase insert size with Alu PCR products amplified from hybrid cell lines containing human chromosome 21, and identified a subset of 63 clones representative of this chromosome. The majority of clones were assigned to chromosome 21 by the presence of specific STSs and in situ hybridization. Twenty-nine of 36 STSs that we tested were detected in the subset, and a contig spanning 20 centimorgans in the genetic map and containing 8 STSs in 4 YACs was identified. The proposed approach can greatly speed efforts to construct physical maps of the human genome.

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