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Isolation and characterization of tricarboxylic acid cycle mutants of Bacillus subtilis.

Authors
  • Carls, R A
  • Hanson, R S
Type
Published Article
Journal
Journal of bacteriology
Publication Date
Jun 01, 1971
Volume
106
Issue
3
Pages
848–855
Identifiers
PMID: 4997541
Source
Medline
License
Unknown

Abstract

A technique was developed for the detection, on agar, of mutants of Bacillus subtilis that lacked a functional tricarboxylic acid cycle. Mutants devoid of detectable levels of aconitase, isocitric dehydrogenase, alpha-ketoglutarate dehydrogenase, succinic dehydrogenase, fumarase, and malate dehydrogenase have been isolated and characterized. Several mutants with conditionally expressible lesions, including a mutant with a heat-sensitive citrate synthase, have also been isolated. All of the mutants examined express all the biochemical markers normally absent in early-stage sporulation mutants except elastase, and some of these mutants sporulated nearly as well as the prototroph.

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