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Isolation and characterization of three new polymyxins in polymyxins B and E by high-performance liquid chromatography.

Authors
  • Elverdam, I
  • Larsen, P
  • Lund, E
Type
Published Article
Journal
Journal of chromatography
Publication Date
Nov 20, 1981
Volume
218
Pages
653–661
Identifiers
PMID: 6274887
Source
Medline
License
Unknown

Abstract

Two polymyxin antibiotics, polymyxins B and E (colistin), have been separated analytically into ten to thirteen components on a commercial reversed-phase material by isocratic elution with a mixture of acetonitrile, phosphate/formate and acetate buffer containing sodium sulphate and triethylamine. The analytical system was transferred to a preparative system, using a C18-bonded stationary phase, without extensive impairing of the selectivity. The major components of each product were isolated and characterized by high-performance liquid chromatography, amino acid analysis and identification of the fatty acid. Three components were isolated and characterized for the first time. The fatty acid was also identified in some of the minor components.

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