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Isolation and characterization of novel thermophilic lipase-secreting bacteria.

Authors
  • Rabbani, Mohammed1
  • Bagherinejad, Mohammad Reza1
  • Sadeghi, Hamid MirMohammad1
  • Shariat, Ziaedin Samsam2
  • Etemadifar, Zahra3
  • Moazen, Fatemeh1
  • Rahbari, Manizheh3
  • Mafakher, Ladan3
  • Zaghian, Saeideh3
  • 1 Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran. , (Iran)
  • 2 Department of Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran. , (Iran)
  • 3 Department of Biology, School of Basic Sciences, University of Isfahan, Isfahan, Iran. , (Iran)
Type
Published Article
Journal
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
Publication Date
Dec 01, 2013
Volume
44
Issue
4
Pages
1113–1119
Identifiers
PMID: 24688500
Source
Medline
Keywords
License
Unknown

Abstract

The purpose of the present study was to screen and identify the lipase-producing microorganisms from various regions of Iran. Samples collected from hot spring, Persian Gulf, desert area and oil-contaminated soil, were analyzed for thermophilic extracellular-lipase producing organisms. Six strains with high activity on rhodamine B plates were selected for chemical identification and further study. Among these isolated bacteria, four strains show higher activity in pH-Stat method at 55 °C. These strains were identified by PCR amplification of 16s rRNA genes using universal primers. Fermentation increased the activity up to 50%. The growth medium, designed for lipase production, increased the activity up to 4.55 folds. The crude supernatant of ZR-5 after fermentation and separation the cells, was lyophilized and the activity was measured. Total activity of this strain was 12 kU/g that shows its potential for industrial uses. Further study is required for purification of enzyme and calculation its specific activity. Immobilization is another approach should be considered.

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