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Isolation and Characterization of a Heparin-Like Compound with Potent Anticoagulant and Fibrinolytic Activity from the Clam Coelomactra antiquata

Authors
  • Du, ZhenXing1, 2, 3
  • Jia, XueJing1, 3
  • Chen, Jing1, 2, 3
  • Zhou, SiYi1, 2, 3
  • Chen, JianPing1
  • Liu, XiaoFei1
  • Cao, XiaoHuang1
  • Zhong, SaiYi1, 2, 3, 4
  • Hong, PengZhi1, 2, 3
  • 1 (P.H.)
  • 2 Shenzhen institute, Guangdong Ocean University, Shenzhen 518108, China
  • 3 Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Zhanjiang 524088, China
  • 4 Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, China
Type
Published Article
Journal
Marine Drugs
Publisher
MDPI AG
Publication Date
Dec 19, 2019
Volume
18
Issue
1
Identifiers
DOI: 10.3390/md18010006
PMID: 31861572
PMCID: PMC7024239
Source
PubMed Central
Keywords
License
Green

Abstract

Heparin from mollusks with unique sulfated glycosaminoglycan exhibits strong anti-thrombotic activities. This study reports on a purified heparinoid from Coelomactra antiquata , which shows potent anticoagulant and fibrinolytic abilities. Its structure was characterized by infrared spectroscopy, high-performance liquid chromatography, and one-dimensional and two-dimensional nuclear magnetic resonance spectroscopy. Its fibrinolytic activity was determined in vitro and in vivo. Its anticoagulant activity was determined by activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT). The results indicated that clam heparinoid was a homogeneous glycosaminoglycan with a molecular weight of 30.99 kDa, mainly composed of →4)-α-IdoA2S-(1→4)-α-GlcNS3S6S (or GlcNS6S)-(1→4)-β-GlcA-(1→4)-α-GlcNS6S (or GlcNAC)-(1→. Furthermore, this heparinoid showed a highly anticoagulant titer and fibrinolytic value of 149.63 IU/mg and 1.96 IU/mg, respectively. In summary, clam heparinoid shows great potential for application in the clinic and antithrombotic drugs industry.

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