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Isolation, characterization and biological activity of a CRF-related diuretic peptide from Periplaneta americana L.

Authors
  • Kay, I
  • Patel, M
  • Coast, G M
  • Totty, N F
  • Mallet, A I
  • Goldsworthy, G J
Type
Published Article
Journal
Regulatory Peptides
Publisher
Elsevier
Publication Date
Dec 11, 1992
Volume
42
Issue
3
Pages
111–122
Identifiers
PMID: 1337794
Source
Medline
License
Unknown

Abstract

A diuretic peptide (Periplaneta-DP) has been isolated from extracts of whole heads of the cockroach, Periplaneta americana. The purified peptide increases cyclic AMP production and the rate of fluid secretion by isolated Malpighian tubules in vitro. In the fluid secretion assay, the response to native Periplaneta-DP is comparable to that obtained with crude extracts of cockroach corpora cardiaca, and the EC50 lies between 10(-8) and 10(-9) M. The primary structure of Periplaneta-DP was established as a 46-residue amidated peptide: T G S G P S L S I V N P L D V L R Q R L L L E I A R R R M R Q S Q D Q I Q A N R E I L Q T I-NH2. Periplaneta-DP is a further member of the recently established family of CRF-related insect diuretic peptides.

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