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[Isolation and characteristics of catalytic antibodies to DNA in systemic lupus erythematosis].

Authors
Type
Published Article
Journal
Biokhimii︠a︡ (Moscow, Russia)
Publication Date
Volume
58
Issue
2
Pages
313–318
Identifiers
PMID: 8485221
Source
Medline

Abstract

A method for purifying DNA-specific catalytic antibodies based on affinity chromatography on protein A Sepharose and on both modified and non-modified DNA-cellulose as well as HPLC has been developed. The elution conditions with high yields of DNA-hydrolyzing activity of antibodies have been optimized. The biochemical and immunological properties of catalytic antibodies have been examined. The kinetic parameters of the enzyme interaction with an oligonucleotide substrate have been determined. The influence of effectors on DNA hydrolysis by antibodies has been investigated.

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