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Isolation of anti-nucleosome antibodies from the plasma of lupus nephritis patients

Authors
  • Suenaga, R.1
  • Mitamura, K.1
  • Abdou, N. I.1
  • 1 St Luke's Hospital, Immunology Research Laboratory, 4401 Wornall Road, Kansas City, Missouri, 64111, USA , Kansas City
Type
Published Article
Journal
Clinical Rheumatology
Publisher
Springer-Verlag
Publication Date
May 01, 1998
Volume
17
Issue
3
Pages
189–194
Identifiers
DOI: 10.1007/BF01451045
Source
Springer Nature
Keywords
License
Yellow

Abstract

Anti-nucleosome antibodies, which recognise conformational epitopes consisting of histone and DNA in chromatin, have been described in autoimmune diseases. In this study, an attempt was made to isolate anti-nucleosome antibodies from the anti-DNA-depleted plasma IgG of two lupus patients either with or without nephritis by nucleohistone affinity chromatography. The purified nucleohistone-binding antibodies bound to nucleohistone in a specific manner and contained enriched anti-histone antibodies. However, adsorption of the purified antibodies with histone revealed that the nephritis patient-derived antibodies contained nucleohistone-specific antibodies. Although such purified antibodies may not recognise native structures of nucleosomes, this chromatography may provide a method to isolate and determine the fine specificity of anti-nucleosome antibodies in various autoimmune diseases.

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