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Isolation and partial characterization of an antibody enhancing factor from leukaemic owl monkey cell cultures.

Authors
  • A B MacDonald
  • C E Fraser
  • A S Rubin
Publication Date
Jan 01, 1978
Source
PMC
Keywords
Disciplines
  • Biology
License
Unknown

Abstract

Herpesvirus saimiri inoculated into owl monkeys (Aotus trivirgatus) causes leukaemia and lymphoma. Peripheral lymphocytes from leukaemic monkeys grown in culture are predominantly T lymphocytes. The supernatants from these cultures contain a factor which enhances the antibody response of murine B cells to sheep red blood cells (SRBC). The factor has been partially characterized by ammonium sulphate precipitation, DEAE-chromatography, gel filtration over Sephadex G-150 and disc-gel electrophoresis. The enhancing factor is a protein with a molecular weight of approximately 40,000.

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