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Isolation and Characterization of the Neutral Leucine Aminopeptidase (LapN) of Tomato1

Authors
  • Chao-Jung Tu
  • Sang-Youl Park
  • Linda L. Walling
Publisher
American Society of Plant Biologists
Publication Date
May 01, 2003
Source
PMC
Keywords
Disciplines
  • Biology
License
Unknown

Abstract

Isolation and Characterization of the Neutral Leucine Aminopeptidase (LapN) of Tomato by Chao-Jung Tu, Sang-Youl Park, and Linda L. Walling Plantphysiol Volume 132(1):243-255 May 1, 2003 ©2003 by American Society of Plant Biologists Specificity of LAP-A domain-specific antibodies. Tu C et al. Plantphysiol 2003;132:243-255 ©2003 by American Society of Plant Biologists Accumulation of LAP-A, LAP-N and LAP-like proteins in vegetative organs. Tu C et al. Plantphysiol 2003;132:243-255 ©2003 by American Society of Plant Biologists Accumulation of LAP-A, LAP-N and LAP-like proteins in cotyledons. Tu C et al. Plantphysiol 2003;132:243-255 ©2003 by American Society of Plant Biologists Accumulation of LAP-A, LAP-N and LAP-like proteins in reproductive organs. Tu C et al. Plantphysiol 2003;132:243-255 ©2003 by American Society of Plant Biologists Genomic DNA-blot analysis and RNase protection studies. Tu C et al. Plantphysiol 2003;132:243-255 ©2003 by American Society of Plant Biologists Alignment of the deduced amino acid sequences of plant LAPs. The deduced amino acid sequence of the tomatoLapN was aligned with LeLapA1,LeLapA2, and LeTPP24 from tomato (Milligan and Gasser, 1995; Gu et al., 1996a),StLAP from potato (Hildmann et al., 1992),... Tu C et al. Plantphysiol 2003;132:243-255 ©2003 by American Society of Plant Biologists Characterization of the His6-LAP-N enzyme expressed in E. coli. Tu C et al. Plantphysiol 2003;132:243-255 ©2003 by American Society of Plant Biologists Specificity of LAP-A domain-specific antibodies. A, Four GST-LapN fusion genes containing domains A, B, D, and F were constructed and overexpressed in E. coli. Black bars, LapA1-coding regions in the 1.9-kb full-lengthLapA1 cDNA clone pBLapA1 (Gu et al., 1996a), the partial LapA1 cDNA clone pDR57 corresponding toLapA1 nucleotides 325 to 1,843 (Pautot et al., 1993), and the GST-LapA fusion constructs. The glutathione-S-transferase (GST)-LAP-A fusion proteins contained the following amino acid residues: A (

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