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Isolated plant nuclei as mechanical and thermal sensors involved in calcium signalling.

Authors
  • Xiong, Tou Cheu
  • Jauneau, Alain
  • Ranjeva, Raoul
  • Mazars, Christian
Type
Published Article
Journal
The Plant journal : for cell and molecular biology
Publication Date
Oct 01, 2004
Volume
40
Issue
1
Pages
12–21
Identifiers
PMID: 15361137
Source
Medline
License
Unknown

Abstract

Calcium signals in the nucleus elicit downstream effects that are distinct from those of cytosolic calcium signals. In the present work, we have evaluated the ability of plant nuclei to sense stimuli directly and to convert them into calcium changes. We show that individual mechanical stimulation of isolated nuclei elicits a single calcium transient at acidic pHs, whereas a series of stimulations leads to oscillations whose frequency reflects that of the stimuli. Conversely, at alkaline pHs, nuclei respond to temperature but not to stretch. The stretch- and the temperature-activated processes differ by their sensitivity to pharmacological drugs known to affect ion channel activities in animal cells. Our data demonstrate that isolated nuclei are able to gauge physical parameters of their environment. This might have a profound influence on the functioning of calcium-dependent processes known to control a large array of molecular events in the nucleus.

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