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Ischemia Reperfusion Injury Triggers CXCL13 Release and B-Cell Recruitment After Allogenic Kidney Transplantation

Authors
  • Kreimann, Kirill1
  • Jang, Mi-Sun1
  • Rong, Song1
  • Greite, Robert1
  • von Vietinghoff, Sibylle1
  • Schmitt, Roland1
  • Bräsen, Jan Hinrich2
  • Schiffer, Lena1
  • Gerstenberg, Jessica1
  • Vijayan, Vijith3
  • Dittrich-Breiholz, Oliver4
  • Wang, Li1
  • Karsten, Christian M.5
  • Gwinner, Wilfried1
  • Haller, Hermann1
  • Immenschuh, Stephan3
  • Gueler, Faikah1
  • 1 Department of Nephrology, Hannover Medical School (MHH), Hannover , (Germany)
  • 2 Nephropathology Unit, Institute of Pathology, Hannover Medical School (MHH), Hannover , (Germany)
  • 3 Institute for Transfusion Medicine, Hannover Medical School (MHH), Hannover , (Germany)
  • 4 Research Core Unit Genomics, Hannover Medical School, Hannover , (Germany)
  • 5 Institute for Systemic Inflammation Research, University of Lübeck, Lübeck , (Germany)
Type
Published Article
Journal
Frontiers in Immunology
Publisher
Frontiers Media SA
Publication Date
Aug 06, 2020
Volume
11
Identifiers
DOI: 10.3389/fimmu.2020.01204
PMID: 32849490
PMCID: PMC7424013
Source
PubMed Central
Keywords
License
Unknown

Abstract

Ischemia reperfusion injury (IRI) is linked with inflammation in kidney transplantation (ktx). The chemokine CXCL13, also known as B lymphocyte chemoattractant, mediates recruitment of B cells within follicles of lymphoid tissues and has recently been identified as a biomarker for acute kidney allograft rejection. The goal of this study was to explore whether IRI contributes to the up-regulation of CXCL13 levels in ktx. It is demonstrated that systemic levels of CXCL13 were increased in mouse models of uni- and bilateral renal IRI, which correlated with the duration of IRI. Moreover, in unilateral renal IRI CXCL13 expression in ischemic kidneys was up-regulated. Immunohistochemical studies revealed infiltration of CD22+ B-cells and, single-cell RNA sequencing analysis a higher number of cells expressing the CXCL13 receptor CXCR5, in ischemic kidneys 7 days post IRI, respectively. The potential relevance of these findings was also evaluated in a mouse model of ktx. Increased levels of serum CXCL13 correlated with the lengths of cold ischemia times and were further enhanced in allogenic compared to isogenic kidney transplants. Taken together, these findings indicate that IRI is associated with increased systemic levels of CXCL13 in renal IRI and ktx.

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