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iRFP is a sensitive marker for cell number and tumor growth in high-throughput systems.

Authors
  • Hock, Andreas K
  • Lee, Pearl
  • Maddocks, Oliver Dk
  • Mason, Susan M
  • Blyth, Karen
  • Vousden, Karen H
Type
Published Article
Journal
Cell Cycle
Publisher
Landes Bioscience
Publication Date
Jan 01, 2014
Volume
13
Issue
2
Pages
220–226
Identifiers
DOI: 10.4161/cc.26985
PMID: 24200967
Source
Medline
Keywords
License
Unknown

Abstract

GFP and luciferase are used extensively as markers both in vitro and in vivo although both have limitations. The utility of GFP fluorescence is restricted by high background signal and poor tissue penetrance. Luciferase throughput is limited in vitro by the requirement for cell lysis, while in vivo, luciferase readout is complicated by the need for substrate injection and the dependence on endogenous ATP. Here we show that near-infrared fluorescent protein in combination with widely available near-infrared scanners overcomes these obstacles and allows for the accurate determination of cell number in vitro and tumor growth in vivo in a high-throughput manner and at negligible per-well costs. This system represents a significant advance in tracking cell proliferation in tissue culture as well as in animals, with widespread applications in cell biology.

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