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Ion and ion channel involvement in alpha-melanocyte-stimulating hormone secretion from superfused slices of rat hypothalamus.

Authors
  • Tiligada, E
  • Wilson, J F
Type
Published Article
Journal
Neuroscience Letters
Publisher
Elsevier
Publication Date
Dec 19, 1988
Volume
95
Issue
1-3
Pages
318–322
Identifiers
PMID: 2852321
Source
Medline
License
Unknown

Abstract

Release of alpha-melanocyte-stimulating hormone (alpha-MSH) from 250 micron frontal slices of rat hypothalamus superfused at 37 degrees C with oxygenated artificial cerebrospinal fluid (ACSF) was quantified in freeze-dried samples of ACSF by radioimmunoassay. Significant reproducible increases in alpha-MSH release were caused by 40-70 mM K+ in ACSF, maximum release being caused by 50 mM K+. Fifty mM K+-stimulated alpha-MSH release was abolished in the absence of Ca2+ from ACSF and by the presence of 10(-6) M tetrodotoxin. Tetrapentylammonium ions, 10(-4) M and 10(-3) M, stimulated dose-dependent increases in alpha-MSH release. The data support a putative neurotransmitter/neuromodulator role for alpha-MSH in the CNS.

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