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Involvement of TNFα in the enhancement of hypersensitivity in the adulthood-injured face associated with facial injury in infancy.

Authors
  • Soma, Kumi1
  • Shinoda, Masamichi2
  • Hayashi, Yoshinori2
  • Kanno, Kohei3
  • Shirakwa, Tetsuo1
  • Iwata, Koichi4
  • 1 Departments of Pediatric Dentistry, School of Dentistry, Nihon University, Tokyo Japan. , (Japan)
  • 2 Departments of Physiology, School of Dentistry, Nihon University, Tokyo Japan. , (Japan)
  • 3 Departments of Endodontics, School of Dentistry, Nihon University, Tokyo Japan. , (Japan)
  • 4 Departments of Physiology, School of Dentistry, Nihon University, Tokyo Japan. Electronic address: [email protected] , (Japan)
Type
Published Article
Journal
Neuroscience research
Publication Date
Dec 01, 2020
Volume
161
Pages
18–23
Identifiers
DOI: 10.1016/j.neures.2019.12.014
PMID: 31917166
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

To evaluate the mechanisms underlying acceleration of hypersensitivity in the adulthood-injured face following facial injury in infants, we developed the rats model with facial skin injury in infants and adulthoods (incision + incision), and facial skin suture in infants and facial skin injury in adulthoods (sham + incision), and analyzed the mechanical head-withdrawal threshold (MHWT) of the facial skin, immunohistochemical analysis of trigeminal ganglion (TG) and the effects of intra-ganglionic administration of neutralizing ant-TNFα antibody and recombinant TNFα on nocifensive behavior. The MHWT became considerably lower in incision + incision rats than in sham + incision rats at 10-14 days after the surgery. We observed many TG neurons encircled by glial fibrillary acidic protein-immunoreactive (GFAP-IR) cells and those exhibited TNFα immunoreactivity. TNFα was also expressed in GFAP-IR cells in incision + inicision TG. TNFα protein levels and the relative number of TNFα-IR cells were significantly higher in incision + incision rats than in sham + incision rats. The MHWT was significantly recovered during the intra-ganglionic administration of neutralizing anti-TNFα antibody 4-14 days after the incision. Furthermore, the MHWT was significantly decreased in sham + incision rats following the intra-ganglionic administration of recombinant TNFα. The present findings suggest that the neuron-satellite glial cell communication via TNFα is a critical mechanism in the enhancement of mechanical hypersensitivity in the adulthood-injured face following facial injury in infants. Copyright © 2020 Elsevier B.V. and Japan Neuroscience Society. All rights reserved.

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