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Involvement of CsWRKY70 in salicylic acid-induced citrus fruit resistance against Penicillium digitatum

Authors
  • Deng, Bing1
  • Wang, Wenjun1
  • Ruan, Changqing1, 2
  • Deng, Lili1, 2
  • Yao, Shixiang1, 2
  • Zeng, Kaifang1, 2
  • 1 Southwest University, Chongqing, 400715, China , Chongqing (China)
  • 2 Research Center of Food Storage & Logistics, Southwest University, Chongqing, 400715, China , Chongqing (China)
Type
Published Article
Journal
Horticulture Research
Publisher
Nature Publishing Group UK
Publication Date
Oct 01, 2020
Volume
7
Issue
1
Identifiers
DOI: 10.1038/s41438-020-00377-y
Source
Springer Nature
License
Green

Abstract

Penicillium digitatum causes serious losses in postharvest citrus fruit. Exogenous salicylic acid (SA) can induce fruit resistance against various pathogens, but the mechanism remains unclear. Herein, a transcriptome-based approach was used to investigate the underlying mechanism of SA-induced citrus fruit resistance against P. digitatum. We found that CsWRKY70 and genes related to methyl salicylate (MeSA) biosynthesis (salicylate carboxymethyltransferase, SAMT) were induced by exogenous SA. Moreover, significant MeSA accumulation was detected in the SA-treated citrus fruit. The potential involvement of CsWRKY70 in regulating CsSAMT expression in citrus fruit was studied. Subcellular localization, dual luciferase, and electrophoretic mobility shift assays and an analysis of transient expression in fruit peel revealed that the nucleus‐localized transcriptional activator CsWRKY70 can activate the CsSAMT promoter by recognizing the W-box element. Taken together, the findings from this study offer new insights into the transcriptional regulatory mechanism of exogenous SA-induced disease resistance in Citrus sinensis fruit.

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