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The involvement of the Candida glabrata trehalase enzymes in stress resistance and gut colonization.

Authors
  • Van Ende, Mieke1, 2
  • Timmermans, Bea1, 2
  • Vanreppelen, Giel1, 2
  • Siscar-Lewin, Sofía3
  • Fischer, Daniel3
  • Wijnants, Stefanie1, 2
  • Romero, Celia Lobo1, 2
  • Yazdani, Saleh1, 2
  • Rogiers, Ona1, 2, 4, 5
  • Demuyser, Liesbeth1, 2
  • Van Zeebroeck, Griet1, 2
  • Cen, Yuke1, 2
  • Kuchler, Karl6
  • Brunke, Sascha3
  • Van Dijck, Patrick1, 2
  • 1 Laboratory of Molecular Cell Biology, Department of Biology, Institute of Botany and Microbiology , Leuven, KU Leuven, Belgium. , (Belgium)
  • 2 VIB-KU Leuven Center for Microbiology , Leuven, Belgium. , (Belgium)
  • 3 Department of Microbial Pathogenicity Mechanisms, Hans Knöll Institute , Jena, Germany. , (Germany)
  • 4 Department of Internal Medicine and Pediatrics, Ghent University , Ghent, Belgium. , (Belgium)
  • 5 VIB-UGent Center for Inflammation Research , Ghent, VIB, Belgium. , (Belgium)
  • 6 Medical University of Vienna, Center for Medical Biochemistry, Max Perutz Labs Vienna, Campus Vienna Biocenter , Vienna, Austria. , (Austria)
Type
Published Article
Journal
Virulence
Publisher
Landes Bioscience
Publication Date
Dec 01, 2021
Volume
12
Issue
1
Pages
329–345
Identifiers
DOI: 10.1080/21505594.2020.1868825
PMID: 33356857
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Candida glabrata is an opportunistic human fungal pathogen and is frequently present in the human microbiome. It has a high relative resistance to environmental stresses and several antifungal drugs. An important component involved in microbial stress tolerance is trehalose. In this work, we characterized the three C. glabrata trehalase enzymes Ath1, Nth1 and Nth2. Single, double and triple deletion strains were constructed and characterized both in vitro and in vivo to determine the role of these enzymes in virulence. Ath1 was found to be located in the periplasm and was essential for growth on trehalose as sole carbon source, while Nth1 on the other hand was important for oxidative stress resistance, an observation which was consistent by the lower survival rate of the NTH1 deletion strain in human macrophages. No significant phenotype was observed for Nth2. The triple deletion strain was unable to establish a stable colonization of the gastrointestinal (GI) tract in mice indicating the importance of having trehalase activity for colonization in the gut.

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