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Involvement of Aquaporins in the Intense Pulsed Light-Enhanced Wound Healing in Diabetic Rats.

Authors
  • Huang, Po-Wei1, 2, 3
  • Jeng, Seng-Feng4, 5
  • Liu, Chi-Ming6
  • Chen, Chien-Chung5
  • Chang, Li-Ren5
  • Shih, Hsiang-Shun5
  • Chen, Hsing-Fu5
  • Yang, Chih-Hui7
  • Chen, Jian-An5, 8
  • Feng, Guan-Ming5
  • 1 Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung, 804, Taiwan. , (Taiwan)
  • 2 Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung, 804, Taiwan. , (Taiwan)
  • 3 Division of Urology, Department of Surgery, Zuoying Branch of Kaohsiung Armed Forces General Hospital, Kaohsiung, 813, Taiwan. , (Taiwan)
  • 4 Institute of Biotechnology and Chemical Engineering, I-Shou University, Kaohsiung, 840, Taiwan. , (Taiwan)
  • 5 Department of Plastic Surgery, E-DA Hospital, Kaohsiung, 824, Taiwan. , (Taiwan)
  • 6 Division of General Surgery, Department of Surgery, Cheng Hsin General Hospital, Taipei, 112, Taiwan. , (Taiwan)
  • 7 Department of Biological Science and Technology, I-Shou University, Kaohsiung, 824, Taiwan. , (Taiwan)
  • 8 Division of Plastic Surgery, Department of Surgery, Kaohsiung Armed Forces General Hospital, Kaohsiung, 802, Taiwan. , (Taiwan)
Type
Published Article
Journal
Lasers in Surgery and Medicine
Publisher
Wiley (John Wiley & Sons)
Publication Date
Apr 01, 2021
Volume
53
Issue
4
Pages
549–556
Identifiers
DOI: 10.1002/lsm.23303
PMID: 32757279
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

We previously demonstrated that intense pulsed light (IPL) irradiation prior to wounding improved the wound healing in rats with diabetes mellitus (DM). Also, we found that IPL upregulated the expression of aquaporin 3 (AQP3), a protein that is crucial for wound healing, in normal rats. This present study aimed to examine the involvement of AQPs in the IPL-enhanced wound healing in diabetic rats. Streptozotocin was used to induce diabetes in Sprague-Dawley rats. Animals were divided into four groups: normal group, DM only group, DM rats with IPL treatment 2 weeks before wounding (DM + IPL-Pre group), and DM rats with concurrent IPL irradiation and wounding (DM + IPL-Con group). Wounds were created on the dorsal skin of rats. The expressions of AQP1, 3, 4, 7, and 9 in the pre-injured skin, periwound, and wound were determined. Among all the AQPs analyzed, only the expressions of AQP3 and AQP7 were significantly altered. Unirradiated diabetic rats showed much higher expression level of AQP3 in the regenerating skin compared with normal rats. IPL pretreatment, but not concurrent treatment, attenuated the expression toward the level detected in the normal wounds. In contrast, a lower expression level of AQP7 was noted in the regenerating skin of DM only rats and IPL pretreatment upregulated the expression to a level similar to that in the normal rats. The beneficial effect of IPL pretreatment on the wound healing in diabetic rats might involve a mechanism by which the expression of AQPs is regulated. Lasers Surg. Med. © 2020 Wiley Periodicals LLC. © 2020 Wiley Periodicals LLC.

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